We find that NeuriteTracer accurately measures neurite outgrowth from cerebellar, DRG and hippocampal neurons. Given user-defined thresholds, the plugin counts neuronal nuclei, and traces and measures neurite length. The plugin analyzes fluorescence microscopy images of neurites and nuclei of dissociated cultured neurons. To automate these measurements, we have written NeuriteTracer, a neurite tracing plugin for the freely available image-processing program ImageJ.

Generally, these measurements are obtained through labor-intensive manual or semi-manual tracing of images. The quantification of these assays requires accurate measurements of neurite length and neuronal cell numbers in neuronal cultures. In vitro assays to measure neuronal growth are a fundamental tool used by many neurobiologists studying neuronal development and regeneration. HIGH-THROUGHPUT, HIGH CONTENT SCREENING.High-Content Screening with AgileOptix Technology.PROTEIN DETECTION, QUANTITATION, ANALYSIS.

NUCLEIC ACID (DNA/RNA) DETECTION & ANALYSIS.

Time lapse tool activation code